Table 1.
Primers used for construction of virus mutants
| Construct | Sequence (5′–3′)a | Direction |
|---|---|---|
| ΔRL | GTATGTGTGGGAGAAAGTATGTCGATTTTAAATGTAGTTGGTCCTGTATCTACCTATAGGTAGGGATAACAGGGTAATCGATTT | Forward |
| ΔRL | CCAATAACTCGAACGCTCTTCCTATAGGTAGATACAGGACCAACTACATTTAAAATCGACGCCAGTGTTACAACCAATTAACC | Reverse |
| IRL deletion | CGAACGGAATGTACAACAGCTTGC | Forward sequencing |
| IRL deletion | GATAAGACACTTTCCCACTCATAC | Reverse sequencing |
| ΔMetvIL-8 | GCAGGGGGTGTGGGTTTGATGAGCAGTTGGGGCGGCAAAATTGCAGGCGTTGTTGCTAGTATTGGTAGGGATAACAGGGTAATCGATTT | Forward |
| ΔMetvIL-8 | CAAATAGATCTGTACTATGAATAGAACCAATACTAGCAACAACGCCTGCAATTTTGCCGCCCCAACTGCTCATCGCCAGTGTTACAACCAATTAACC | Reverse |
| ΔMetvIL-8rev | TGTACTATGAATAGAACCAATACTAGCAACAACGCCTGCATTTTTGCCGCCCCAACTGCTAGTGTTACAACCAATTAACC | Forward |
| ΔMetvIL-8rev | GCAGGGGGTGTGGGTTTGATGAGCAGTTGGGGCGGCAAAAATGCAGGCGTTGTTGCTAGTGATAACAGGGTAATCGATTT | Reverse |
| vIL-8 seq | CTGCTATGCAGGGGTCGTGGGAA | Forward sequencing |
| vIL-8 seq | GCACCTCTTGTCGACAGCGAGAC | Reverse sequencing |
a
Mutated sequences are shown in bold letters.