Fig 1.

Differentiation of CD14⁺ monocytes with TGF-β promotes a Langerhans-like phenotype. (A) Freshly isolated monocytes were characterized for CD14⁺, CD83⁻, and CD207⁻ expression. (B) Light microscopy of freshly isolated monocytes or monocytes cultured for 6 days in IL-4/GM-CSF or IL-4/GM-CSF/TGF-β to promote the formation of a DC (MoDC)- or an LC (MoLC)-like cell type. (C) MoDCs, MoLCs, or Langerhans cells generated from CD34⁺ precursors (CD34⁺ LCs) were analyzed by flow cytometry for class I, class II, CD1a, CD207, and E-Cadherin expression.