Fig 4.

Mature MoLCs support the complete replicative cycle of HCMV. (A) Mature MoLCs were either mock (lanes M) or TB40/e (lanes V) infected and then analyzed by Western blotting for IE, gB, and GAPDH expression at 24 and 72 h postinfection (hpi). (B and C) Mature CD14⁺ derived DCs (MoDC) or LCs (MoLC) were infected with TB40/e and then analyzed every 2 days for virus production in the supernatant. Alternatively, at 7 days postinfection both the supernatant (cell-free) and the lysed cells (cell-associated) were assayed for infectious virus.