Fig 7.

Efficient reactivation of HCMV from CD34⁺ derived LCs is maturation dependent and is responsive to IL-6. (A) CD34⁺ derived immature LCs either mock treated (column 1) or cultured with LPS (column 2), IL-6 at 5 to 50 ng/ml (columns 3 and 5), or LPS plus IL-6 at 5 to 50 ng/ml (columns 4 and 6) and then analyzed for IE and actin gene expression by real-time quantitative RT-PCR. The results are expressed as the fold change in IE gene expression compared to LPS alone. (B) CD34⁺ derived immature LCs were either mock treated (column 1) or incubated with LPS (column 2), LPS plus neutralizing IL-6 antibody (IL6; columns 3, 5, and 7), or isotype-matched control (IgG; columns 4, 6, and 8) and analyzed by quantitative RT-PCR for IE and actin gene expression. Reactivation is expressed as a function of LPS alone (100%).