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. 2012 Aug;86(16):8507–8515. doi: 10.1128/JVI.00598-12

Fig 8.

Fig 8

Reactivation of naturally latent HCMV is observed in LCs derived from CD14+ and CD34+ progenitor cells. (A) CD14+ cells isolated from CMV-seropositive and -seronegative donors were differentiated to immature LCs and then either mock treated (lane 1) or incubated with LPS (lane 2), LPS plus 50 ng of IL-8/ml (lane 3), or LPS plus 50 ng of IL-6/ml (lane 4) and analyzed for IE and actin gene expression by RT-PCR (n = 4). (B) CD34+ cells isolated from CMV-seropositive and -seronegative donors were differentiated to immature LCs and then either mock treated (lane 1) or incubated with LPS (lane 2), LPS plus 1 μg of nIL6ab/ml (lane 3), LPS plus 1 μg of nIL8ab/ml (lane 4), LPS plus 50 ng of IL-6/ml (lane 5), or LPS plus 50 ng of IL-8/ml (lane 6) and then analyzed for IE and actin gene expression by RT-PCR (n = 4).