Table 4.
Frankliniella occidentalis proteins differentially expressed between tomato spotted wilt virus-exposed and nonexposed first-instar larvae
| Finding and spot no. | P value from ANOVA | Fold change | Database searched (sequence matched) | Mascot score | Protein ID | Identified motifs | E value |
|---|---|---|---|---|---|---|---|
| Upregulated in response to TSWV infection | |||||||
| 201 | 0.0046 | 1.22 | Fo Seq (CL4589Contig1_S454) | 625 | Triosephosphate isomerase | TIM_phosphate binding | 5e−110 |
| NCBI (gi|259016078) | 151 | Triosephosphate isomerase | TIM_phosphate binding | 4e−41 | |||
| 47a | 0.0257 | 1.22 | Fo Seq (CL2263Contig1_S454) | 422 | Myosin 3 light chain | Ca+2 binding site, EFh | 2e−15 |
| NCBI (gi|312371061) | 334 | Hypothetical protein AND_22684 (similar to Skp1) | Skp1 | 4e−80 | |||
| 836a | 0.0326 | 1.57 | Fo Seq (CL63Contig1_S454) | 488 | Similar to voltage-dependent anion-selective channel 2 | Porin3, Porin3_Tom40 | 6e−118 |
| NCBI (RS3_AMBME) | 124 | 40S ribosomal protein S3 | 40S_S3_KH, KH-II, Ribosomal_S3_C | 1e−160 | |||
| 137 | 0.0355 | 1.54 | Fo Seq (CL4310Contig1_S454) | 264 | Mitochondrial ATP synthase α subunit | ATPase_alpha | 0.0 |
| NCBI (gi|52630965) | 261 | Mitochondrial ATP synthase α subunit | ATPase_alpha | 0.0 | |||
| 271a | 0.0433 | 1.12 | Fo Seq (CL4116Contig1_S454) | 499 | cxpwmw03 (similar to electron transfer flavoprotein subunit alpha) | ETF_alpha, AANH-like | 3e−153 |
| NCBI (gi|328786330) | 129 | Saccharopine dehydrogenase-like isoform 1 | GTP_cyclohydrol, TFold, NADB-Rossmann | 0.0 | |||
| 374a | 0.0508 | 1.69 | Fo Seq (contig16594) | 284 | Vitellogenin | lipoprotein N-terminal | 5e−113 |
| NCBI (gi|156763) | 155 | Actin | Actin | 0.0 | |||
| 277 | 0.0551 | 1.28 | Fo Seq (CL3352Contig1_S454) | 215 | Alpha-tocopherol transfer protein-like isoform 1 | SEC14 | 1e−164 |
| NCBI (no match) | |||||||
| 134 | 0.0616 | 1.36 | Fo Seq (CL2397Contig1_S454) | 557 | Vacuolar proton ATP synthase subunit E | V-ATP_synt-E | 4e−71 |
| NCBI (gi|46561760) | 300 | Vacuolar proton ATP synthase subunit E | V-ATP_synt-E | 7e−110 | |||
| 161 | 0.0662 | 1.40 | Fo Seq (CL4706Contig1_S454) | 782 | AGAP007827-PA isoform 1 (similar to enolase) | Enolase-like | 0.0 |
| NCBI (gi|157121051) | 434 | Enolase | Enolase-like | 0.0 | |||
| 72a | 0.0668 | 1.21 | Fo Seq (CL1656Contig1_S454) | 956 | Glutathione S-transferase | Thioredoxin-like, GST_Sigma | 4e−52 |
| NCBI (gi|66565444) | 149 | 60S ribosomal protein L9 | ribosomal_L6 | 2e−101 | |||
| Downregulated in response to TSWV infection | |||||||
| 244 | 0.0090 | 2.05 | Fo Seq (contig19201) | 389 | Cytochrome b-c1 complex subunit 2 | peptidase_M16, peptidase M16_C | 2e−103 |
| NCBI (no match) | |||||||
| 481a | 0.0128 | 1.50 | Fo Seq (contig15458) | 323 | Apolipoprotein D | lipocalin_2 | 4e−84 |
| NCBI (K1C10_HUMAN) | 534 | Keratin type I cytoskeletal 10 | Filament | 0.0 | |||
| 310 | 0.0338 | 1.62 | Fo Seq (no match) | ||||
| NCBI (no match) | |||||||
| 88 | 0.0466 | 1.26 | Fo Seq (CL423Contig1_S454) | 401 | Predicted hypothetical protein (similar to rad23) | RAD_23, UBA, XPC binding | 1e−77 |
| NCBI (gi|242023622) | 169 | UV excision repair protein rad23 | RAD_23, UBA, XPC binding | 3e−164 | |||
| 794 | 0.0460 | 1.29 | Fo Seq (contig30771) | 214 | |||
| NCBI (LYSC_CHICK) | 263 | Lysozyme C | LYZ1, Lys | 6e−110 | |||
| 378a | 0.0486 | 1.95 | Fo Seq (CL1213Contig1_S454) | 495 | AGAP009685-PA (similar to aspartate aminotransferase) | AAT_I | 0.0 |
| NCBI (gi|156763) | 215 | Actin | Actin | 0.0 | |||
| 319 | 0.0548 | 1.28 | Fo Seq (contig17796) | 232 | Peroxiredoxin 1-like | Thioredoxin-like | 5e−83 |
| NCBI (gi|58377838) | 88 | Thioredoxin-dependent peroxidase | Thioredoxin-like | 2e−111 | |||
| 819a | 0.0589 | 2.42 | Fo Seq (CL827Contig1_S454) | 360 | Zinc finger protein 1 | ZPR1_znf, zf-ZPR1, ZPR1 | 0.0 |
| NCBI (PDK3_HUMAN) | 264 | Pyruvate dehydrogenase kinase 3 | HATPase_C, BCDHK_Adom3, Bae5, Vick | 0.0 | |||
| 340a | 0.0597 | 1.92 | Fo Seq (CL2403Contig1_S454) | 523 | Hypothetical protein SINV_09553 (similar to stress-induced phosphoprotein 1) | Tad D, TPR | 0.0 |
| NCBI (gi|170027766) | 137 | Electron transfer flavoprotein; ubiquinone oxidoreductase | Pyr_Redox | 0.0 | |||
| 430a | 0.0667 | 1.38 | FoSeq (CL862Contig1_S454) | 425 | Phospholipid hydroperoxide glutathione peroxidase | Thioredoxin-like, GSH_peroxidase | 2e−67 |
| NCBI (gi|242008321) | 243 | Conserved hypothetical protein (similar to actin depolymerization factor 1) | ADF | 1e−81 | |||
| 196a | 0.0763 | 1.12 | Fo Seq (CL4854Contig1_S454) | 512 | Cyclophilin | cyclophilin_ABH, CLD | 6e−83 |
| NCBI (gi|307643755) | 228 | Glyceraldehyde-3-phosphate dehydrogenase | Gp_dh_C | 3e−109 | |||
| 803 | 0.0787 | 1.43 | Fo Seq (CL18Contig1_S454) | 362 | Heat shock protein 70 cognate 4 | HSP70, Dnak, HscA | 0.0 |
| NCBI (HSP7C_BOVIN) | 218 | Heat shock cognate 71 protein | HSP70, Dnak, HscA | 0.0 | |||
| 283a | 0.0833 | 1.29 | Fo Seq (contig16705) | 625 | Protein yellow-like | MRJP | 2e−138 |
| NCBI (gi|157134067) | 248 | 26S protease regulatory subunit 7 | P-loop NTPase | 3e−162 | |||
| 296 | 0.0851 | 1.55 | FoSeq (contig14504) | 240 | Arginine kinase | phosphagen_ kinase | 2e−129 |
| NCBI (gi|161088212) | 227 | Arginine kinase | phosphagen_ kinase | 4e−130 | |||
| 206 | 0.0997 | 1.15 | FoSeq (contig14504) | 640 | Arginine kinase | phosphatase_kinase | 1e−103 |
| NCBI (gi|161088180) | 496 | Arginine kinase | phosphatase_kinase | 1e−104 | |||
| 182 | 0.0998 | 1.35 | Fo Seq (CL3105Contig1_S454) | 422 | Glyceraldehyde-3-phosphate dehydrogenase | Gp_dh_C/N | 3e−101 |
| NCBI (gi|156547538) | 435 | Glyceraldehyde-3-phosphate dehydrogenase | Gp_dh_C/N | 2e−173 |
a
Different proteins were identified within a single spot using the Fo Seq and the Metazoan nr protein database from NCBI.