Fig 2.

(A) Growth curve analysis of Kunjin and yellow fever viruses with increasing concentrations of BG-323. BHK cells were infected with Kunjin or yellow fever virus at an MOI of 0.01, and the indicated concentration of BG-323 was added at time zero. Medium samples were collected at the indicated times and stored at −80°C. Samples virus titers were determined by plaque assay. Curves were generated in Prism. n = 3. (B) Sindbis virus is insensitive to BG-323 treatment. BHK cells were infected with a Renilla luciferase-expressing Sindbis virus (pBG451) (24) at an MOI of 0.01, and BG-323 was added at the indicated concentrations. The Renilla luciferase (white bars) and CellTiter-Glo (black bars) signal levels measured are shown in relative light units (RLU). n = 3.