Table 1.
Macromolecular properties derived from SAXS and light-scattering measurements
| Sample |
Rg (Å) |
Dmaxa (Å) | Porod vol (Å3)b | Rh (DLS)c (Å) | Mass (kDa) |
||
|---|---|---|---|---|---|---|---|
| Guinier | GNOMa | Measuredd | Expectede | ||||
| gp120 | 37.1 ± 0.03 | 37.6 ± 0.1 | 130 | 230,374 | 46.5 ± 0.4 | 98.1 ± 13.0 | 93 |
| gp120−sCD4 | 37.0 ± 0.05 | 38.0 ± 0.1 | 135 | 246,073 | 48.3 ± 0.4 | 106.8 ± 6.0 | 113.4 |
| gp120−sCD4-17b | 42.8 ± 0.06 | 43.5 ± 0.1 | 150 | 299,816 | 54.2 ± 0.8 | 149.7 ± 16.5 | 162.1 |
| gp120 dimer | 47.0 ± 0.05 | 47.9 ± 0.1 | 166 | 449,665 | 52.7 ± 0.9 | 168.1 ± 33.4 | 186 |
| sCD4 (D1D2) | 20.9 ± 0.05 | 21.7 ± 0.2 | 73 | 25,216 | 23.8 ± 0.2 | 18.9 ± 5.8 | 20.3 |
| 17b Fab | 26.7 ± 0.03 | 26.5 ± 0.2 | 90 | 63,701 | 31.5 ± 0.2 | 49.1 ± 6.2 | 48.7 |
a
Real space Rg and Dmax values from GNOM (65).
b
Porod were volumes determined using Autoporod (47).
c
Hydrodynamic radii (Rh) were obtained from DLS measurements.
d
Value is the mean molecular mass determined from SLS and by two SAXS methods; individual values are presented in Table S1 of the supplemental material.
e
The expected MW was calculated based on the most predominant glycoforms observed during HDX-MS analysis.