Fig 2.

Bcl-2 and Beclin1 associate in HCV-infected hepatocytes. (A and B) IHH were infected with HCV, and cell lysates prepared after 3 days of infection were immunoprecipitated with mouse monoclonal Bcl-2 or rabbit anti-Beclin1 antibody. Mock-infected IHH were treated similarly as control. Interaction of Beclin1, Vps34, or Bcl-2 was detected by immunoblotting with specific antibody. (C) Cells were transfected with Bcl-2 and flag-tagged Beclin1 plasmid DNAs in the presence or absence of FL-HCV. Cell lysates prepared after 2 days of transfection were immunoprecipitated with a mouse monoclonal antibody to Bcl-2. The interaction of Beclin1 or Bcl-2 was detected by immunoblotting with specific antibody. (D) Control and nutrient-starved (for 4 h) cell lysates were prepared for immunoprecipitation with a mouse monoclonal antibody to Bcl-2. The interaction of Beclin1 or Bcl-2 was detected by immunoblotting with specific antibody.