Fig 3.

HCV infection induces p110β but does not associate with Beclin1 or Vps34. (A) IHH were infected with HCV, and infected cell lysates were prepared 3 days postinfection. Cell lysates were analyzed for expression of p110β by Western blotting with specific antibody. Mock-infected IHH were treated similarly to the control. The blot was reprobed with an antibody to actin for a comparison of the protein load. (B) IHH were infected with HCV, and cell lysates were immunoprecipitated with anti-p110β antibody. Mock-treated IHH were used as a control in parallel. The interaction of p110β with Beclin1 and Vps34 was examined by immunoblotting with anti-Beclin1 or anti-Vps34 antibody. (C) HCV-infected cell lysates were analyzed for activation of MEK/ERK by Western blot analysis. Mock-treated IHH were used as a control in parallel. The blot was reprobed with an antibody to total MEK1/2. The blot was also reprobed with an antibody to tubulin for comparison of the protein load in each lane.