Table 1.
Silent-infected-cell estimation from coincubation experiments
| Target cell | Repeat (donor) | Separate incubationa |
% Coincubation double positiveb | Infection potency (%)c |
|||
|---|---|---|---|---|---|---|---|
| % Reporter 1 positive | % Reporter 2 positive | Reporter 2/1 ratio | Reporter 1 | Reporter 2 | |||
| T CD3+ | 1 | 7.3 ± 0.1 | 5.8 ± 1.5 | 0.8 ± 0.2 | 1.7 ± 0.6 | 14.6 | 11.7 |
| 2 | 5.3 ± 0.0 | 3.0 ± 0.1 | 0.6 ± 0.0 | 0.9 ± 0.0 | 12.7 | 7.1 | |
| HPB | 1 | 7.8 ± 0.1 | 3.8 ± 0.3 | 0.5 ± 0.0 | 2.9 ± 0.0 | 24.1 | 11.9 |
| 2 | 5.3 ± 0.4 | 2.2 ± 0.0 | 0.4 ± 0.0 | 1.4 ± 0.0 | 18.0 | 7.6 | |
| 3 | 5.0 ± 0.4 | 4.0 ± 0.4 | 0.8 ± 0.1 | 4.9 ± 0.4 | 24.9 | 19.8 | |
| HeLa | 1 | 6.5 ± 0.4 | 4.5 ± 0.3 | 0.7 ± 0.1 | 0.8 ± 0.3 | 11.0 | 7.6 |
| 2 | 6.2 ± 0.1 | 4.7 ± 0.0 | 0.8 ± 0.0 | 1.0 ± 0.1 | 11.5 | 8.7 | |
| 3 | 5.8 ± 0.2 | 4.2 ± 0.2 | 0.7 ± 0.0 | 1.1 ± 0.2 | 12.1 | 8.8 | |
| HeLad | 1 | 6.5 ± 0.4 | 2.8 ± 0.1 | 0.4 ± 0.0 | 0.9 ± 0.0 | 14.3 | 6.2 |
| 2 | 6.2 ± 0.1 | 3.8 ± 0.3 | 0.6 ± 0.1 | 0.9 ± 0.1 | 12.4 | 7.6 | |
| 3 | 5.8 ± 0.2 | 2.6 ± 0.1 | 0.4 ± 0.0 | 0.9 ± 0.1 | 14.5 | 6.5 | |
a
Cells were incubated separately with viruses encoding either reporter 1 or reporter 2; the percentages of reporter-positive cells measured by flow cytometry and the reporter 2/reporter 1 ratio are indicated ± standard deviation (SD).
b
Cells were incubated with both viruses, and the percentages of double-positive cells are indicated ± SD.
c
Theoretical percentages of reporter-positive cells upon separate incubation were calculated from double-positive cells as described in Materials and Methods.
d
Cells were incubated with reporter 1 and/or 24 h later with reporter 2.