Fig 2.
Comparative functional analysis of the effects of APH-2 and HBZ on the repression of Tax-mediated transcription. 293T cells (1.5 × 105 cells) were cotransfected with 1 μg HTLV or negative-control plasmid DNA, 0.1 μg LTR-Luc reporter, 0.01 μg TK-Renilla, and various amounts (0.05, 0.1, and 0.2 μg) of the HBZ or APH-2 expression vector. All transfections were performed in duplicate and normalized to TK-Renilla to control for the transfection efficiency. Cell lysates were harvested 48 h after transfection. Black bars represent luciferase reporter activities for HTLV-1 and LTR-1-Luc, and gray bars represent luciferase reporter activities for HTLV-2 and LTR-2-Luc. RLU, relative light units.