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APH-2 protein expression in stably transfected cell lines. A total of 6 × 107 cells of each stably transfected cell line (729.HTLV-2, 729.HTLV-2APHΔBD2, and 729.HTLV-2ΔAPH) and the control 729 cell line were lysed and subjected to IP using a rabbit anti-APH-2 polyclonal antibody and subjected to WB analysis using an APH-2-specific antiserum. Lysates from 293T cells transfected with APH and APHΔBD2 cDNA plasmids are presented as positive controls for protein expression and mobility.
