Table 1.

Sequence identities (percent) for 192-bp wzx gene sequences from nonpneumococcal species and from carriage specimens that coamplify with targeted PCR assay for pneumococcal sg10F/10C^h

Sequence	% identity
Sequence	Spn 10F^a	Spn 33C^b	Spn 35B^c	S. infantis SS1641^d	S. gordonii SS1245^d	S. salivarius SS1061^d	S. oralis SS911, SS1236, and 32^e^,^f	Specimen 49^f	Specimen 248^f	Specimen 265^f	Specimen 300^f
Spn 10F^a		88.0	87.0	87.0	92.7	92.2	92.7	92.7	93.2	92.7	93.2
Spn 33C^b			90.1	90.6	94.3	93.2	95.3	93.2	93.8	93.2	93.2
Spn 35B^c				95.8	89.1	90.6	89.1	90.1	90.6	91.1	91.1
S. infantis SS1641^d					90.6	89.6	89.6	89.6	90.1	90.6	90.6
S. gordonii SS1245^d						97.4	99.0	97.9	98.4	97.9	97.9
S. salivarius SS1061^d							97.4	97.4	97.9	97.4	96.4
32^f								97.9	98.4	97.9	97.9
49^f									99.5	99.0	97.9
248^f										99.5	98.4
265^f											97.9
83^g	100

Only available pneumococcal serotype 10F reference wzx sequence (GenBank accession no. CR931652), from which mPCR assay primers derived (192 bp after subtracting mPCR primer sequences) (2), which is also identical to the serotype 10C reference mPCR amplicon sequence (GenBank accession no. CR931651) .

The corresponding pneumococcal serotype 33C wzx sequence (192 bp, from GenBank accession no. cr931700).

The corresponding pneumococcal serotype 35B wzx sequence (192 bp, from GenBank accession no. cr931705).

These sequences are available at the CDC Streptococcus Lab website at http://www.cdc.gov/ncidod/biotech/strep/pcr.htm (see PCR deduction protocols, point 3).

These 3 amplicons shared sequence identity and were also identical within their overlap with GenBank submission AB289547 describing the S. oralis surface polysaccharide synthetic gene cluster. All included amplicons were of the exact same length of 248 bp.

Sequences corresponding to amplicons representative of multiple carriage specimens PCR positive for sg10F/10C. Specimens either were lytA negative or were positive for confirmed pneumococcal isolates of nonrelated serotypes. Sequences corresponding to specimens 49, 248, and 300 are available at the CDC Streptococcus Lab website. Specimens 32 and 265 are identical within their overlaps with recent S. oralis GenBank submissions AB289547 and FR720602, respectively.

Sequence corresponds to amplicon from the single carriage specimen that yielded a serotype 10F pneumococcal isolate. Specimen was mPCR positive for 10F/10C and was lytA positive.

Shading indicates corresponding pneumococcal reference sequences for serotypes 10F, 33C, and 35B. Spn, S. pneumoniae.