Table 2.

Sequence identities (percent) for suspected nonpneumococcal 507-bp wzy gene sequences from carriage specimens that coamplify with targeted PCR assay for pneumococcal sg18A/18B/18C/18F with pneumococcal references 18A, 18C, and 18F^e

Sequence(s)	% identity
	18Aa	18Ca	18Fa	257b	262b	269b	368b
18A		97.6	97.8	89.5	90.5	92.1	90.7
18C			99.0	89.3	90.7	91.9	90.9
18F				90.3	90.9	92.5	91.1
257					92.1	92.7	91.9
262						96.3	99.4
269							96.4
8, 33, 103, 323c	100
117, 369, 395d		100

^aThe indicated 507-bp reference amplicon sequences (after subtracting primer sequences [2]) are within GenBank accessions CR931671 (18A), CR931673 (18C), and CR931674 (18F). The 18C and 18B reference amplicons share the identical 507-bp sequence. All amplicons in the table are of the same length (573 bp).

^bSequence corresponds to mPCR amplicon for sg18A/18B/18C/18F from indicated carriage specimen. Specimens either were lytA negative or were positive for confirmed pneumococcal isolates of non-sg18 serotypes. These sequences are available in GenBank as JX104734 (specimen 257), JX105735 (specimen 262), JX105736 (specimen 269), and JX105737 (specimen 368).

^cSequence corresponding to amplicons representative of carriage specimens PCR positive for sg18A/18B/18C/18F. Specimens were lytA positive and yielded serotype 18A pneumococcal isolates.

^dSequences corresponding to amplicons representative of carriage specimens PCR positive for sg18A/18B/18C/18F. Specimens were lytA positive and yielded serotype 18C pneumococcal isolates.

^eShading indicates pneumococcal references 18A, 18C, and 18F.