Table 1.
Antibiotic | Initial concn (μg/ml) | MIC (μg/ml) |
||||
---|---|---|---|---|---|---|
RMC26 |
CT31-7d |
S. Typhimurium LT2 | ||||
MEP | MVA | MEP | MVA | |||
Ampicillin | 200 | 2.5 | 2.5 | NA | NA | 0.8–1.6 |
Carbenicillin | 200 | 20 | 200 | NA | NA | 100 |
Streptomycin | 200 | 50 | 100 | 100 | 100 | 12.5 |
Neomycin | 200 | NA | NA | NA | NA | 25 |
Azithromycin | 20 | 0.31–2.5 | 5–10 | 5 | 5 | 12.5 |
Clindamycin | 200 | 0.16–0.63 | 2.5–5 | NA | NA | 2.5–5 |
Erythromycin | 200 | 50–100 | 100–200 | 25–50 | 50 | 100–200 |
Clarithromycin | 200 | 25–50 | 100 | 25–50 | 50 | 100–200 |
Lincomycin | 200 | 12.5 | NA | 25–50 | 200 | NA |
Tetracycline | 2 | 0.25–0.5 | 0.5–2 | 2 | 2 | 0.5 |
Doxycycline | 20 | 0.08–0.6 | 0.63–1.25 | 1.25–2.5 | 1.25 | 0.5 |
Trimethoprim | 20 | 2.5 | 20 | 1.25–2.5 | 1.25–2.5 | 0.5 |
Chloramphenicol | 20 | 1.25 | 2.5 | 1.25–2.5 | 1.25–2.5 | 5 |
Ciprofloxacin | 0.08 | 0.005–0.01 | 0.01–0.02 | 0.01 | 0.01 | 0.02 |
Levofloxacin | 2 | 0.06–0.25 | 0.25–0.5 | 0.03 | 0.03 | 0.03 |
Nalidixic acid | 20 | 1.25 | 2.5 | 2.5 | 2.5 | 1.25–5 |
Fosfomycin | 100 | (-) | (-) | 1 | 1 | (-) |
Fosmidomycin | Var. | 0.2 | NA | 0.01 | NA | 10 |
MICs were determined as the lowest concentration that inhibited bacterial growth > 90%, as determined by A600 values relative to no-inhibitor control results. A minimum of 3 independent experiments, each run in duplicate, were used to determine MICs. Plates were incubated at 37°C and 80% humidity with shaking at 250 rpm. Plates were read after 18 h. NA = no activity; (-) = not screened.