Fig 6.

Real-time PCR of three putative azithromycin efflux pumps. MAC was incubated with 16 mg/liter azithromycin. (A) MAV_1659 demonstrated no significant upregulation during the first 72 h of exposure to azithromycin. (B) MAV_1406 demonstrated no significant change at 1 h (1.36- ± 0.30- versus 1.01- ± 0.17-fold) but was upregulated at 24 h (56.05- ± 9.99- versus 1.01- ± 0.17-fold) compared to baseline. There was no further change in induction beyond 24 h. (C) MAV_3306 induction was stepwise, starting at 1 h (1.68- ± 0.46- versus 1.00- ± 0.46-fold) to 50.60- ± 9.41- versus 1.01- ± 0.11-fold at 72 h. The error bars indicate SD.