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Quenching efficiency of the fluorescence emitted by the tryptophan residue of bovicin HC5 by spin-labeled lipids (TEMPO-PC, 5DOX-PC, and 12DOX-PC) incorporated at 25 mol% in DOPC (■) or DLPC/DMoPC (□) vesicles containing 1 mol% of lipid II. Single-wavelength recordings were performed at 340 nm using an excitation wavelength of 280 nm. The quenching efficiencies were calculated from the tryptophan fluorescence in the presence of membranes with or without spin-labeled lipids (n = 5).
