Table 1.

Substitutions in the Cyp51A protein, EUCAST MICs, and cyp51A sequence genotypes of 13 A. fumigatus sSNP isolates recovered from nine patients

No. of sSNP isolates	Substitutions in Cyp51A proteina	MIC range (μg/ml)f			Patient no.	Gtb	Microsatellite markerc				GCd
		ITC	VRC	PSC			A	B	C	D
8	F46Y, M172V, E427K	0.5–1	0.25–0.5	0.125	6	89	150	110	170	72	GC1
					1	90	154	110	170	72	GC1
					7	90	154	110	170	72	GC1
					8	91	154	110	172	72	GC1
					3	92	154	118	172	72	GC1
					9	93	158	110	170	72	GC1
					9	94	158	110	170	74	GC1
					5	79	130	114	172	72	Se
1	F46Y, M172V, N248T, E427K	0.5	0.25	0.0625	3	77	126	118	164	92	Se
3	F46Y, M172V, N248T, D255E, E427K	1	0.25–0.5	0.125	2	76	124	158	164	94	GC2
					4	78	126	158	164	94	GC2
					5	78	126	158	164	94	GC2
1	F46Y, M172V, N248T, D255E				1	26	104	128	118	96	Se

^aThe substitutions t18c, c283t, t489a (F46Y), c560t, c619t, g690a (M172V), a937g, and a1497g SNPs in the cyp51A gene were common to all isolates, while a1166c (N248T), c1188g (D255E), g1702 (E427K), and t1785c were inconsistently found.

^bGt, genotype. The values correspond to the genotypes of the isolates and are arbitrary.

^cThe values correspond to the lengths in base pairs of the PCR products of the four microsatellite markers (A to D) and are a function of the number of repeats of the microsatellite at each locus.

^dThe GCs were determined for all isolates using MST, PCA, and UPGMA clustering. GC1 and GC2 were genetically distinct from WT isolates using these three analyses.

^eThe isolates that had more than one allelic mismatch with the other isolates were considered singletons (S).

^fITC, itraconazole; VRC, voriconazole; PSC, posaconazole.