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. 2012 Sep;56(9):4640–4649. doi: 10.1128/AAC.00623-12

Fig 2.

Fig 2

CLDs inhibit HIV-1 capture and transfer by Raji/DC-SIGN cells and iMDDCs. BaL Env-pseudotyed HIV-1 was preincubated with or without inhibitor for 1 h at 37°C before the addition to Raji/DC-SIGN cells or iMDDCs. Cells pretreated with or without mannan were exposed to viruses as controls. After exposure to virues for 2 h at 37°C, cells were extensively washed and either lysed for capture assay or cocultured with U87-CD4.CCR5 cells for transfer assay. (a) HIV-1 captured by Raji/DC-SIGN cells. Medium alone was defined as 100% and its p24 concentration was 1.79 ng/ml. (b) RLU of HIV-1 trans infection from Raji/DC-SIGN cells to U87-CD4.CCR5 cells. Medium alone was defined as 100%. (c) HIV-1 captured by iMDDCs. Medium alone was defined as 100% and its p24 concentration was 2.87 ng/ml. (d) RLU of HIV-1 trans infection from iMDDCs to U87-CD4.CCR5 cells. Medium alone was defined as 100%. Data shown are mean ± SD of three independent experiments, with each condition performed in triplicate.