Fig 1.

PI(4,5)P2 accumulates in discrete patches in irs4 and inp51 mutants. C. albicans SC5314 (wild-type) and irs4 and inp51 mutants expressing CaPH-GFP were grown in yeast (A to C, respectively; YPD medium at 30°C) or hyphal (D to F, respectively; YPD medium + 5% fetal bovine serum [FBS] at 37°C) morphologies in 35-mm-diameter glass-bottom dishes and imaged using confocal microscopy. As shown, CaPH-GFP was distributed uniformly in SC5314 (A and D) but concentrated in discrete patches in irs4 (B and E) and inp51 (C and F) mutants.