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PI(4,5)P2 and chitin colocalize at aberrant patches in irs4 and inp51 mutants. PI(4,5)P2 was localized in C. albicans SC5314 and irs4 and inp51 mutants using a CaPH-GFP reporter (A, D, and G, respectively), as in the experiments shown in Fig. 1. Chitin was localized in the same cells by calcofluor white staining (B, E, and H, respectively). Overlay images revealed that CaPH-GFP and calcofluor white colocalized to aberrant sites in irs4 (F) and inp51 (I) mutants (cyan color).
