Fig 7.

C. albicans irs4 and inp51 mutants display cell wall damage response gene expression profiles that are similar to those of septin regulatory protein kinase mutants. The expression of 14 damage response genes was analyzed in mutant strains in the absence of cell wall stress. The expression of TDH3, a housekeeping gene involved in glycolysis, was used to normalize expression between strains. Strains were further normalized to their complemented strain to reduce strain background errors. Resultant values were log base 2 transformed. As shown, irs4 and inp51 mutants were firmly positioned within the septin regulatory protein kinase functional group by hierarchical analysis and clustered most tightly with gin4. The cell wall-related protein kinase mutant ire1 was an outlier from the other mutants. Blue and yellow on heat map refer to log base 2 transformed values of −2 and 2, respectively.