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The gin4 mutant (A) demonstrates aberrant chitin and PI(4,5)P2-containing patches. CaPH-GFP (B) and calcofluor white (C) colocalized in aberrant patches in C. albicans gin4 mutant cells (D, cyan color and arrowheads in overlay image), similarly to irs4 and inp51 mutants. gin4 mutants were created using the SAT-flipper method and C. albicans SC5314.
