Table 1.
C. albicans strains
| Strain | Parent | Description | Research purpose | Reference |
|---|---|---|---|---|
| SC5314 | C. albicans reference strain | Wild type | 15 | |
| irs4_2KO | SC5314 | irs4Δ/irs4Δ | irs4 null mutant | This study |
| irs4_2KO_Reins | irs4_2KO | Complemented, irs4Δ/IRS4 | IRS4 reinsertion | This study |
| inp51_2KO | SC5314 | inp51Δ/inp51Δ | inp51 null mutant | 3 |
| inp51_2KO_Reins | Inp51_2KO | Complemented, inp51Δ/INP51 | INP51 reinsertion | 3 |
| gin4_2KO* | SC5314 | gin4Δ/gin4Δ | gin4 null mutant | This study |
| gin4_2KO_Reins* | gin4_2KO | Complemented, irs4Δ/IRS4 | GIN4 reinsertion | This study |
| SC_AGPH | SC5314 | RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| irs4_AGPH | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| inp51_AGPH | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| gin4_AGC10 | gin4_2KO | gin4Δ/gin4Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| SC_AGC10 | SC5314 | RP10/rp10::CDC10-GFP | CDC10-GFP expression | This study |
| irs4_AGC10 | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::CDC10-GFP | CDC10-GFP expression | This study |
| inp51_AGC10 | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::CDC10-GFP | CDC10-GFP expression | This study |
| SC_AGS7 | SC5314 | RP10/rp10::SEP7-GFP | SEP7-GFP expression | This study |
| irs4_AGS7 | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::SEP7-GFP | SEP7-GFP expression | This study |
| inp51_AGS7 | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::SEP7-GFP | SEP7-GFP expression | This study |
| CAI_R5G | CAI4 | ura3::imm434/ura3::imm434 iro1/iro1::imm434, Rbt5sig.GFP.GPI | RBT5-GFP expression | 31 |
| rs4_AGR5 | CAI_R5G | Same as parent, irs4Δ/irs4Δ | RBT5-GFP expression | This study |
| inp51_AGR5 | CAI_R5G | Same as parent, inp51Δ/inp51Δ | RBT5-GFP expression | This study |
| SC_AGPHRC10 | SC_AGPH | RP10/rp10::CaPHx2-GFP, CDC10-RFP | PH-GFP and CDC10-RFP expression | This study |
| irs4_AGPHRC10 | irs4_AGPH | irs4Δ/irs4Δ, RP10/rp10::CaPHx2-GFP, CDC10-RFP | PH-GFP and CDC10-RFP expression | This study |
| inp51_AGPHRC10 | inp51_AGPH | inp51Δ/inp51Δ, RP10/rp10::CaPHx2-GFP, CDC10-RFP | PH-GFP and CDC10-RFP expression | This study |
| gin4_AGPHRC10 | gin4_ AGPH | gin4Δ/gin4Δ, RP10/rp10::CaPHx2-GFP, CDC10-RFP | PH-GFP and CDC10-RFP expression | This study |
| SC_AGPHRS7 | SC_AGPH | RP10/rp10::CaPHx2-GFP, SEP7 | PH-GFP and SEP7-RFP expression | This study |
| irs4_ AGPHRS7 | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::CaPHx2-GFP, SEP7 | PH-GFP and SEP7-RFP expression | This study |
| inp51_ AGPHRS7 | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::CaPHx2-GFP, SEP7 | PH-GFP and SEP7-RFP expression | This study |
| SC_AGV4 | SC5314 | RP10/rp10::Vrg4-GFP | VRG4-GFP expression | This study |
| irs4_ AGV4 | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::Vrg4-GFP | VRG4-GFP expression | This study |
| inp51_ AGV4 | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::Vrg4-GFP | VRG4-GFP expression | This study |
| SC_S1SPG | SC5314 | RP10/rp10::SAP1_SP-GFP | SAP1-GFP (secretory) expression | This study |
| irs4_ S1SPG | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::SAP1_SP-GFP | SAP1-GFP (secretory) expression | This study |
| inp51_ S1SPG | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::SAP1_SP-GFP | SAP1-GFP (secretory) expression | This study |
| SC_AGV4 | SC5314 | RP10/rp10::Arf1-GFP | ARF1-GFP expression | This study |
| irs4_ AGV4 | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::Arf1-GFP | ARF1-GFP expression | This study |
| inp51_ AGV4 | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::Arf1-GFP | ARF1-GFP expression | This study |
| SC_S1SPG | SC5314 | RP10/rp10::Arf2-GFP | ARF2-GFP expression | This study |
| irs4_ S1SPG | irs4_2KO | irs4Δ/irs4Δ, RP10/rp10::Arf2-GFP | ARF2-GFP expression | This study |
| inp51_ S1SPG | inp51_2KO | inp51Δ/inp51Δ, RP10/rp10::Arf2-GFP | ARF2-GFP expression | This study |
| BWP17 | C. albicans reference strain (auxotropically marked) | 59 | ||
| DAY185 | BWP17 | C. albicans reference strain (auxotrophies corrected) | Wild-type control | 59 |
| DAY_AGPH | DAY185 | RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| cdc10−/− (YAW7) | BWP17 | cdc10Δ/cdc10Δ | cdc10 null mutant | 55, 56 |
| cdc11−/− (YAW11) | BWP17 | cdc10Δ/cdc10Δ | cdc10 null mutant | 55, 56 |
| ckb2−/− (JJH351) | BWP17 | ckb2Δ/ckb2Δ | ckb2 null mutant | 6 |
| CKB2−/−/+ | ckb2Δ/ckb2Δ | Complemented, ckb2Δ/CKB2 | CKB2 reinsertion | 6 |
| ckb2_AGPH | ckb2Δ/ckb2Δ | ckb2Δ/ckb2Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| cla4−/− (JJH262) | BWP17 | cla4Δ/cla4Δ | cla4 null mutant | 6 |
| CLA4−/−/+ | cla4Δ/cla4Δ | Complemented, cla4Δ/CLA4 | CLA4 reinsertion | 6 |
| cla4_AGPH | cla4Δ/cla4Δ | cla4Δ/cla4Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| gin4−/− (JJH276) | BWP17 | gin4Δ/gin4Δ | gin4 null mutant | 6 |
| GIN4−/−/+ | gin4Δ/gin4Δ | Complemented, gin4Δ/GIN4 | GIN4 reinsertion | 6 |
| gin4_AGPH | gin4Δ/gin4Δ | gin4Δ/gin4Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| hsl1−/− (JJH377) | BWP17 | hsl1Δ/hsl1Δ | hsl1 null mutant | 6 |
| HSL1−/−/+ | hsl1Δ/hsl1Δ | Complemented, hsl1Δ/HSL1 | HSL1 reinsertion | 6 |
| hsl1_AGPH | hsl1Δ/hsl1Δ | hsl1Δ/hsl1Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| kin3−/− (JJH381) | BWP17 | kin3Δ/kin3Δ | kin3 null mutant | 6 |
| KIN3−/−/+ | hsl1Δ/hsl1Δ | Complemented, kin3Δ/KIN3 | KIN3 reinsertion | 6 |
| kin3_AGPH | hsl1Δ/hsl1Δ | kin3Δ/kin3Δ, RP10/rp10::CaPHx2-GFP | PH-GFP expression | This study |
| tpk1−/− (JJH384) | BWP17 | tpk1Δ/tpk1Δ | tpk1 null mutant | 6 |
| TPK1−/−/+ (JJH233) | tpk1Δ/tpk1Δ | Complemented, tpk1Δ/TPK1 | TPK1 reinseriton | 6 |
| bck1−/− (JJH256) | BWP17 | bck1Δ/bck1Δ | bck1 null mutant | 6 |
| BCK1−/−/+ (JJH246) | bck1Δ/bck1Δ | Complemented, bckΔ/BCK1 | BCK1 reinsertion | 6 |
| ire1−/− (SF008P) | BWP17 | ire1Δ/ire1Δ | ire1 null mutant | 6 |
| IRE1−/−/+ (SF008C) | ire1Δ/ire11Δ | Complemented, ire1Δ/IRE1 | IRE1 reinsertion | 6 |