Fig 5.

The trypan blue staining pattern of the cell line subjected to CSRB (A and B) and RB (C and D) treatment with and without photodynamic treatment (PDT). The cells subjected to CSRB showed the presence of photosensitizer (pink) within the cytoplasm (arrow) of the viable cells with clear round nuclei (A). RB did not show any uptake into the cells and was found outside as dark pink aggregates (arrow) (C). Following PDT, cells from both groups showed increased uptake of trypan blue uptake (nuclei of cells are stained dark bluish-purple). However, RB plus PDT (D) resulted in disruption of cell morphology and an irregular cell membrane, which was less in cells subjected to CSRB plus PDT (fibroblast cells with extensions) (B).