Fig 2.

Two-dimensional cluster analysis of S. aureus fitness test profiles. Actinocarbasin, krisynomycin, and M131(R,S-lysine) were compared to three compounds of known mechanism of action, amoxicillin, the translation inhibitor GE2270, and roxithromycin. Antisense strains that are significantly growth inhibited by the compound treatment are shown in magenta, and strain resistances are shown in cyan. The thresholds for including strains in the cluster analysis are a >5-fold reduction in strain abundance and a P value of ≤0.01 in three or more experiments. The profiles of strains that are reduced by actinocarbasin, krisynomycin, or M131(R,S-lysine) treatment cluster tightly together and are characterized by significant depletions of the spsB antisense strain at all compound concentrations tested.