Frequency of apoptotic and clonogenic cells in taxol- or taxol and 4-HT-treated LAN-1 (parental, p53-72R, or p53-72P) cells. (A) Western blot shows levels of p53 Ser-15 phosphorylation in LAN-1 (parental, p53-72R, or p53-72P) cells treated with taxol or etoposide (positive control) and 4-HT. (B) Histograms show percent apoptosis (hypodiploid DNA content). (C) Residual colony formation after treatment of LAN-1 (parental, p53-72R, p53-72) cells with taxol or taxol and 4-HT. Results are presented as the mean ± SD of three (B) or two (C) experiments performed with three different seeding (C). NS indicates not significant, P > .05.