Table 1.
Growth characteristics of coleoptiles grown in normoxic (aerated), hypoxic (3% O2), and anoxic (N2) solution Coleoptiles were collected at 3, 4, and 5 d after imbibition and ethanol-insoluble dry weights (EiDW) were determined. Protein and free amino acid levels were determined by amino acid hydrolysis and water extraction, respectively, and were analysed via ultra-high performance liquid chromatography. Total DNA was extracted with phenol–chloroform and total amounts determined spectrophotometrically. Total lipids were determined by extracting powdered, freeze-dried material with hexane/ethanol (95:5%, v/v). (ND, not determined.) ‘col’ refers to ‘coleoptile’. Data are mean and SEM (n ≥3 for all samples). Statistically different data across treatments (Tukey-Kramer, α=0.05) are indicated by different letters.
| Normoxic | Hypoxic | Anoxic | ||||
| Time (d) | 3 | 4 | 3 | 4 | 3 | 4 |
| Dry weight (mg col−1) | 1.2±0.2 a | 1.4±0.2 a | 0.4±0.1 b | 0.5±0.1 b | 0.3±0.1 b | 0.3±0.1 b |
| Ethanol-insoluble dry | 0.61±0.22 a | 0.92 ±0.21 a | 0.28±0.07 b | 0.31±0.09 b | 0.11±0.04 c | 0.14±0.02 c |
| weight (mg col−1) | ||||||
| Protein | 137±5 a | 142±8 a | 152±13 a | 157±18 a | 138±9 a | 143±11 a |
| (mg g−1 DW) | ||||||
| Free amino acids | 16.1±0.8 b | ND | 62.9±6.8 b | ND | 41.2±2.8 b | ND |
| (mg g-1 DW) | ||||||
| DNA (μg col−1) | 1.2±0.6 a | ND | 1.1±0.3 a | ND | 0.8±0.1 a | ND |
| Lipid | 109±16 a | 93±12 a,b | 122±22 a | 88±9 b | 106±19 a | 81±13 a,b |
| (mg g−1 DW) | ||||||