Haemoglobin effects on Pseudomonas syringae pv. tomato interactions with Arabidopsis. (A) Lesionphenotypes at 48 h post inoculation (hpi) with the virulent disease-forming strain P. syringae pv. tomato DC3000 (Pst) and the avirulent, hypersensitive response (HR)-eliciting strain Pst AvrRpm1 in Col-0, the haemoglobin (Hb) GLB1 RNAi-suppressed Arabidopsis line glb1, and the GLB1 overexpression line 35S-GLB1. Single representative examples are given for Pst AvrRpm1 and Pst-elicited lesions forming in Col-0, but two examples are given for lesions forming in glb1 and 35S-GLB1 to represent the range of phenotypes that were obtained. (B and C) Bacterial populations at 48 hpi of Pst
(B, filled bars) and Pst AvrRpm1 (C, open bars) with from 1-cm-diameter cores sampled from inoculated tissue of Col-0, the glb1 RNAi-suppressed line, the Hb glb2 and glb3 mutants, and the 35S-GLB1 and -GLB2 overexpression lines. Results are mean ± SE (n = 6). Levels of significant difference to results obtained for Col-0 are indicated: NS, no significant difference; * P < 0.05; ** P <0.01; *** P
< 0.001. (D and E) Electrolyte leakage from 1-cm-diameter discs sampled from leaves of Col-0 (filled diamonds), glb1 (filled squares), or 35S-GLB1 (filled triangles) immediately following inoculation with Pst
(D) or
Pst AvrRpm1 (E). Results are mean ± SE (n = 6).