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. 2012 May 10;63(12):4475–4484. doi: 10.1093/jxb/ers123

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 1. — Transgenic CsACS2 tobacco plants. (A) The two plasmid vectors used for tobacco transformation. The CsACS2 coding sequence was digested with BamHI and SacI, and ligated into pBI121 to produce the pBI121-P35S-CsACS2 construct. The CsACS2 promoter fragment was then digested with ClaI and BamHI to give the pBI121-PCsACS2-CsACS2 vector. (B) PCR and dot blotting analysis of transgenic tobacco plants. A total of 36 regenerated plants (27 P35 plants and nine PM plants) were assayed to find the positive transgenic events. Wild-type tobacco is indicated as CK, and this figure revealed part of the positive results. (C) Analysis of CsACS2 expression in transgenic plants. The left panel shows the expression pattern in four P35 plants (T3-6, T3-8, T3-22, and T3-26). No expression was detected in any positive PM transgenic tobacco plants, and the right panel shows the analysis of root, stem, leaf, and bud tissues from the TM-3 plant. (D) Phenotypes of the transgenic tobacco plants. TM-3 was a plant derived from the PM transformation (CsACS2 under control of the cucumber native promoter), and T3-8 was from the transformation of P35 (CsACS2 under control of the 35S promoter). (This figure is available in colour at JXB online.)