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Quantitative RT-PCR of CsACS2 expression in five cucumber lines: G06 (FFMM), M06 (ffMM), G34 (FFMM), H34 (FFmm), and ‘Lemon’ (ffmm). Total RNA was extracted from shoot apices of cucumber plants at the four-leaf stage. Each result was the average of three independent biological replicates, and the significance at P ≤ 0.05 is indicated by mean standard error bars (n=3).
