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. 2012 Jul 20;63(12):4615–4629. doi: 10.1093/jxb/ers137

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© The Author 2012. Published by Oxford University Press. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 8. — Immunodetection of HvPap-1 protease in barley embryos after 24 h of water imbibition. (A) Immunofluorescence localization of HvPap-1 on 2 μm sections of formaldehyde-fixed and LR white-embedded barley embryos 24 hai. Numerous bright foci of different sizes (arrows) are observed within the cytoplasm, excluding the vascular bundle (xy). (B) The fluorescent foci correspond to refringent structures (arrows) revealed by differential interference contrast (DIC). The cell walls (asterisk), clearly identified by DIC, also show some unspecific autofluorescence as seen in the negative control (inset in A).