Table 1.
Inhibition constant (Ki ) values of the barley cystatins and the two propeptides derived from the HvPap-1 protease, against the HvPap-1 barley peptidase expressed as recombinant proteins in E. coli cultures
| Inhibitors | Ki (M) |
| Cystatins | |
| HvCPI-1 | 1.5×10-7 |
| HvCPI-2 | 4.1×10-8 |
| HvCPI-3 | NI |
| HvCPI-4 | 2.8×10-7 |
| HvCPI-5 | 3.5×10-7 |
| HvCPI-6 | 1.3×10-9 |
| HvCPI-7 | NI |
| HvCPI-8 | 6.4×10-7 |
| HvCPI-9 | 1.8×10-8 |
| HvCPI-10 | NI |
| HvCPI-11 | 2.5×10-8 |
| HvCPI-12 | NI |
| HvCPI-13 | 7.3×10-7 |
| Propeptides | |
| HvPap-1pro | 2.3×10-8 |
| HvPap-1pro* | 1.5×10-8 |
No inhibitory activity (NI) was observed at 5 mM concentration of the cystatin.