Table 1. Km values of apoplastic (aPOD), ionic (iPOD), and covalent (cPOD) cell wall-bound peroxidases from pea roots and HRP for different reducing substrates (pyrogallol, chlorogenic, caffeic, and ferulic acids) in the presence of 3.3mM H 2 O 2 K m values for H2O2 were obtained with pyrogallol as a substrate.
| CGA, chlorogenic acid; CA, caffeic acid; FA, ferulic acid. | |||||
| K m (mM) | |||||
| Fraction | H2O2 | Pyrogallol | CGA | CA | FA |
| aPOD | 1.4±0.2 | 2.6±0.4 | 0.6±0.2 | 2.7±0.5 | 4.0±0.7 |
| iPOD | 1.3±0.1 | 2.5±0.3 | 1.0±0.2 | 2.4±0.3 | 1.2±0.3 |
| cPOD | 2.4±0.4 | 2.7±0.3 | 0.7±0.12 | 2.1±0.2 | 0.6±0.2 |
| HRP | 1.4±0.3 | 1.1±0.1 | 1.6±0.3 | 0.9±0.2 | 1.1±0.4 |