Table 2. Catalase-like activity of the cell wall fraction with cPODs, iPODs, soluble extract from pea roots (sol. ex.), and commercial peroxidase from horseradish (HRP). Differences in peroxidase sensitivity on inactivation with H2O2 were determined by measurements of pyrogallol-dependent peroxidase activity as described in the Materials and methods after aliquots of all fractions were incubated for 30min in 10mM H2O2 in phosphate buffer, pH 6.5 at 30 °C. Catalase-like activity was determined by measuring oxygen production with a Clark-type electrode in the presence of 3mM H2O2 at 30 °C.
| cPOD | iPOD | sol. ex. | HRP | |
| O2 evolution (nmol min–1 g–1 protein) | 230±38 | 43±6 | 40±3 | 17±0.6 |
| Inactiation of POD activity (%) | 9±3 | 64±11 | 76±9 | 81±4 |