Fig 2.

Intestinal Gata6 deletion results in a reduction in the proliferation and migration rates in the mature mouse colonic epithelium. (A to D) H&E staining reveals an increase in goblet-like structures in crypts of G6del colon (A and B), a modest (nonsignificant) reduction in crypt depth (C), and significant increase in crypt width (D). (E and F) Transmission EM confirmed an accumulation of goblet-like cells in crypts. (G to K) The number of Ki67-positive cells as a percentage of total epithelial cells is decreased in G6del colon compared to Ctl colon (G to I) and is supported by a decrease in the number of BrdU-positive cells in G6del colon harvested 1 h after BrdU injection (J and K). (L to N) BrdU immunostaining 3 days after BrdU injection revealed a significantly lower percentage of cells at the surface epithelium (n = 4 in each group). *, P < 0.05; **, P < 0.01. Bars: A and B, 100 μm; E and F, 10 μm; G and H, 400 μm; J, K, L, and M, 200 μm.