Fig 7.

GSK-3β deficiency results in increased expression of Nrf2-target genes and antioxidant capacity. (A) Immunoblots showing protein levels of five enzymes regulated by Nrf2 (heme oxygenase 1 [HO-1], glutathione S-transferase M5 [GSTM5], γ-glutamyl cysteine ligase modulatory subunit [GCLM], γ-glutamyl cysteine ligase catalytic subunit [GCLC], and NADPH quinone oxidoreductase [NQO1]). (B) Densitometric quantification of protein levels from representative blots like those in panel A. (C) mRNA levels of HO-1, GSTM5, GCLM, GCLC, and NQO1, by qRT-PCR. (D) Levels of reduced glutathione (GSH). (E) Levels of oxidized glutathione (GSSG). (F) GSH/GSSG ratio. (G) Protein carbonyl content as determined by DNPH levels. (H) Thiobarbituric acid-reactive substances (TBARs) representing mostly MDA. Values are means ± SEM (n = 3). *, P < 0.05.