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. 2012 Sep;32(17):3479–3485. doi: 10.1128/MCB.00389-12

Fig 4.

Fig 4

Histone H3K36 was required for Ioc4 association with chromatin. (A) The levels of Ioc4HA at the 3′ end of the active MET16 gene were measured relative to input by ChIP-qPCR in the indicated strains. Results are shown relative to the WT, which was set to 1. (B) Quantitative immunoblots for TAP-tagged Met16 or HA-tagged Ioc4 in the indicated strains. (C) The levels of Sas3HA at the 5′ end of the COX10 gene were measured relative to input by ChIP-qPCR in the indicated strains. Results are shown relative to the WT. (D) Purified Isw1b was incubated with immobilized mononucleosomes generated from recombinant histones with the indicated methyl-lysine analogs at H3K36. Samples were immunoblotted for TAP-tagged Ioc4 or histone H3 as indicated. Input represents 10% of the Isw1b added to the binding assays. For panels A to C, error bars indicate the means ± SEM from three independent experiments.