Fig 5.

The mammalian m4EHP interaction network. (A) Cytoscape (38) figure displaying the high-confidence interaction partners by AP-MS. The top row represents bait proteins that were tagged and purified. The bottom row shows the protein hits identified by MS. The thickness of the connectors reflects the total number of spectra acquired for each bottom protein. (B) HEK293T cells were triply transfected with HA-4EHP, Flag-ZNF598, and Myc-GIGYF2-WT or Myc-GIGYF2-Mut expression vectors. Interactions were examined by coimmunoprecipitation (Co-IP) with anti-HA, anti-FLAG, and anti-Myc antibodies followed by immunoblotting (IB) with anti-HA, anti-FLAG, and anti-Myc antibodies. Input represents 10% of the total lysate used in the IP assay.