Fig 4.

Recruitment of some (Nrd1 and Pcf11) but not other termination factors (Sen1 and Ssu72) is altered in ess1 mutants. ChIP analysis of four termination factors was performed. No significant effects were detected on recruitment of termination factors (Sen1 and Ssu72) to the intergenic region of TEF2-MUD1 or to the SRG1 ncRNA gene or the PYK1 control gene. An increase in Nrd1 and a decrease in Pcf11 are observed at the 3′ regions of TEF2, SRG1, and RNQ1, similar to previous results for snoRNA loci (51). The inability to bind or release these factors in ess1 mutants may contribute to defective termination of these genes. Data in panels indicated by stars are reprinted from Molecular Cell (51) with permission of the publisher and are reproduced here for completeness.