Fig 6.
Ess1 mutants are synthetic lethal with NMD and RNA decay mutants. Serial dilutions (1:5, starting with cells at an OD600 of 0.5) of the wild type or the indicated mutant cells were grown at 30°C or 35°C on YEPD for 3 days. At both permissive (30°C) and semipermissive (35°C) temperatures, the growth defect of the ess1H164R mutant is strongly enhanced by upf1 (NMD pathway), xrn1 (cytoplasmic nuclease), and rrp6 (nuclear exonuclease) mutations. At 35°C, growth of the double mutants was negligible. Single upf1 mutants have no discernible growth defects, while xrn1 and rrp6 mutants have only minor growth defects at 30°C or 35°C, respectively.