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. 2012 Aug 13;7:4459–4472. doi: 10.2147/IJN.S33575

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© 2012 Zhang et al, publisher and licensee Dove Medical Press Ltd.

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

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Cell adhesion ability assay. Adhesion ability of BMMSCs was analyzed by counting stained nuclei with DAPI under a confocal laser scanning microscope after 4 hours of incubation. The upper panel shows CLSM images of BBMSCs on three substrates (A–C). Statistical results for adhesive cell numbers is shown in the lower panel (D).

Notes: ^##P < 0.01 versus Ti control group; *P < 0.05 versus Ti-6 h group.

Abbreviations: DAPI, 4′, 6-diamidino-2-phenylindole dihydrochloride; Ti, control titanium surface; Ti-6 h, small size nano-sawtooth surface, treated with 30 wt% H₂O₂ for 6 hours; BMMSCs, bone marrow mesenchymal stem cells; CLSM, confocal laser scanning microscope.