Figure 9.

Cell proliferation assay. (A) After one, 4, and 7 days of incubation, the proliferation ability of rat BMMSCs on different substrates was measured by a nuclei counting assay under CLSM. (B) Statistical analysis for nuclei counting assay.

Notes: ^##P < 0.01 versus Ti control group; *P < 0.05 versus Ti-6 h group.

Abbreviations: Ti, control titanium surface; Ti-6 h, small size nano-sawtooth surface, treated with 30 wt% H₂O₂ for 6 hours; BMMSCs, bone marrow mesenchymal stem cells; CLSM, confocal laser scanning microscope.