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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nat Med. 2012 Apr 15;18(5):829–834. doi: 10.1038/nm.2721

Figure 4. Histological validation.

Figure 4

Upper row: 10 µm frozen sections from the margin of an eGFP+U87MG brain tumor were stained for eGFP (green) to visualize the tumor margins and CD11b (red) to visualize glial cells and were examined by laser scanning confocal microscopy. Bottom row: A 50 µm adjacent slice was examined by Raman microscopy to visualize the distribution of the MPRs. Note the Raman signal corresponding to the eGFP+ cells, indicating the presence of the probe in the tumor but not in the adjacent healthy tissue. The Raman signal (red) was scaled from 0 to 100 (AU). Boxes not drawn to scale. STEM images verified the presence of MPRs in the brain tissue, while no MPRs were seen in the healthy brain tissue. A three-dimensional STEM rendering of MPRs in brain tumor is provided in the Supplementary Information as Supplementary Movie.

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