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. 2012 May 17;7:70. doi: 10.1186/1748-717X-7-70

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Copyright ©2012 Sharungbam et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Validation of the gene stability measure (M) and the geometric averaging of carefully selected control genes for normalization. The average gene-specific variation (determined as coefficient of variation in percent) for the three control genes with the smallest variation within each cell line after normalization with three different factors calculated as the geometric mean of the three control genes with the lowest (NF_3(1–3)), intermediate (NF_3(11–13)) and highest (NF_3(30–32)) gene-stability values (as determined by geNorm). The data confirmed the stability of the ECGs. The value under the star indicates the normalization factor for each cell line.