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. 2012 May 17;7:70. doi: 10.1186/1748-717X-7-70

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Copyright ©2012 Sharungbam et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Logarithmic histogram of the expression levels of 32 ECGs in all the cell lines. The 32 ECGs were normalized to the geometric mean of five control genes (RPLPO, UBC, TBP, PPIA and PSMC4). In A431, a 422-fold expression difference is observed between the most stable gene (PGK1) and the least stable gene (18 S), while in A549 and BxPC3, a fold difference of 530 and 375 respectively were observed between them. In addition it shows the cell line specific differences in expression levels of particular genes (e.g. YWHAZ).