Table 1.
Cycle threshold (CT) values and coefficient of variation (CV) of 32 endogenous control genes across the samples of the cell lines
| Gene Symbol | CT Range | CT Min. | CT Max. | Mean CT ± SEM | CV(%) |
|---|---|---|---|---|---|
|
RPL37A |
1.13 |
17.95 |
19.08 |
18.44 ± 0.29 |
1.6 |
|
HMBS |
1.39 |
23.96 |
25.35 |
24.61 ± 0.41 |
1.7 |
|
CASC3 |
1.22 |
22.79 |
24.01 |
23.45 ± 0.42 |
1.83 |
|
RPLP0 |
1.36 |
17.22 |
18.58 |
17.83 ± 0.34 |
1.91 |
|
PSMC4 |
1.56 |
21.04 |
22.6 |
21.90 ± 0.46 |
2.12 |
|
ABL |
1.92 |
22.95 |
24.87 |
23.71 ± 0.51 |
2.15 |
|
UBC |
1.62 |
19.22 |
20.85 |
19.81 ± 0.43 |
2.19 |
|
GAPDH |
1.25 |
16.01 |
17.25 |
16.53 ± 0.37 |
2.27 |
|
POLR2A |
2.03 |
23.5 |
25.53 |
24.19 ± 0.56 |
2.35 |
|
MT-ATP6 |
1.39 |
15.18 |
16.57 |
15.66 ± 0.38 |
2.46 |
|
PES1 |
1.97 |
21.04 |
23.02 |
22.12 ± 0.55 |
2.49 |
|
TBP |
2.19 |
23.88 |
26.08 |
24.47 ± 0.64 |
2.62 |
|
GUSB |
1.7 |
22.28 |
23.98 |
23.30 ± 0.63 |
2.71 |
|
EIF2B1 |
2.53 |
23.593 |
26.12 |
24.11 ± 0.70 |
2.91 |
|
RPS17 |
1.6 |
17.58 |
19.18 |
18.14 ± 0.53 |
2.92 |
|
RPL30 |
1.89 |
17.24 |
19.14 |
18.05 ± 0.53 |
2.96 |
|
POP4 |
1.17 |
22.73 |
23.91 |
23.64 ± 0.72 |
3.05 |
|
PPIA |
2.15 |
17.43 |
19.58 |
18.08 ± 0.55 |
3.07 |
|
PUM1 |
2.88 |
22.15 |
25.03 |
23.10 ± 0.71 |
3.08 |
|
HPRT1 |
2.73 |
21.18 |
23.91 |
22.08 ± 0.68 |
3.1 |
|
CDKN1B |
2.08 |
22.8 |
24.98 |
23.98 ± 0.81 |
3.41 |
|
PGK1 |
2.25 |
18.17 |
20.42 |
19.35 ± 0.69 |
3.61 |
|
MRPL19 |
3.09 |
22.73 |
25.82 |
24.01 ± 0.95 |
3.96 |
|
CDKN1A |
2.6 |
21.3 |
23.99 |
22.98 ± 0.95 |
4.17 |
|
ACTB |
5.65 |
16.1 |
21.75 |
17.80 ± 0.75 |
4.23 |
|
ELF1 |
3.59 |
21.81 |
25.39 |
22.76 ± 1.00 |
4.4 |
|
IPO8 |
4.26 |
23.68 |
27.93 |
24.80 ± 1.13 |
4.57 |
|
GADD45A |
4.2 |
24.37 |
28.57 |
26.29 ± 1.37 |
4.97 |
|
YWHAZ |
3.56 |
22.17 |
25.73 |
23.96 ± 1.26 |
5.27 |
|
TFRC |
4.26 |
21.15 |
25.41 |
22.13 ± 1.18 |
5.37 |
|
B2M |
3.76 |
17.99 |
21.75 |
19.33 ± 1.21 |
6.29 |
| 18 S | 2.95 | 8.79 | 11.95 | 10.78 ± 1.02 | 9.5 |
The genes are sorted by the coefficient of variation increasing from top to bottom.
Four replicates were used for each cell line. Cell lines were irradiated with photon, proton and carbon ions. Non-irradiated samples served as control. Standard error of the mean (SEM).