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. Author manuscript; available in PMC: 2013 May 7.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Mar 30;51(19):4628–4632. doi: 10.1002/anie.201109058

Figure 3.

Figure 3

Gel electrophoresis of ZsGreen1 gene and protein synthesized from the RNA assemblies. A) Agarose gel electrophoresis of the product of RT-PCR amplification of the assembled ZsGreen1 transcript with a ZsGreen1 forward primer and a His-tag-appended ZsGreen1 reverse primer, along with a 100 bp DNA ladder marker (left). The expected size is 714 bp. No RT-PCR product was detected in control experiments without template. B) Electrophoretic analysis in a reducing SDS polyacrylamide gel of the ZsGreen1 protein product obtained in an E. coli cell-free expression system from the gene shown in (A). The expected size is 26.9 kDa. C) Electrophoretic analysis in a nonreducing SDS polyacrylamide gel of the same protein product and standard marker set, as shown in (B). ZsGreen1 exists under nonreducing conditions as a tetramer of theoretical molecular weight 107.6 kDa. It does not migrate true to the expected molecular weight under these nonreducing gel conditions.