FIG. 1.

Isolation, derivation, and characterization of clonal mBMDS cells. A: BM cells (2 × 10⁶ cells/ml) from Balb/c mice were plated and cultured for 2–7 days to obtain the adherent mBMDS cells (top). Cloned mBMDS cells were used for the in vitro differentiation protocol by culturing cells in the presence of a 23-mmol/l glucose concentration for various times. Many cell clusters at various stages of cluster formation during the course of induction of cell differentiation were observed, with a representative shown in the bottom panel. HG, high glucose; LG, low glucose. B: A representative phenotype of the mBMDS cells. FITC, fluorescein isothiocyanate; PE, phycoerythrin.